Georgi Petkov, Ph.D.

Professor

(803) 777- 8988 | CLS 609D

Dr.Petkov's Email

Dr. Georgi Petkov

Research Interest

As a tenured professor of pharmacology in the South Carolina College of Pharmacy (SCCP) with joint appointments at the University of South Carolina (USC) and the Medical University of South Carolina (MUSC), my primary research interest is in the mechanisms that regulate smooth muscle function and the role of membrane ion channels in this cell type. In the past 20 years, my studies have focused on the modulation of excitation-contraction coupling in various types of smooth muscle, including vascular, gastrointestinal and urinary bladder. My past and present research endeavors have employed a wide variety of techniques ranging from subcellular to the whole organism level to elucidate the mechanisms by which a number of physiologically and clinically relevant substances modulate membrane ion channels and smooth muscle function. I have prior training in excellent laboratories in Europe and the US, a long-term record of independent extramural funding, service as an editor and reviewer to numerous journals, and experience in training junior faculty, postdocs, technicians, undergraduate and graduate students.

Currently, I am a standing member of the former NIH UGPP Study Section (currently ZRG1 DKUS-G 90) and serve on several Editorial Boards of journals in the field of physiology, pharmacology, and urology, including American Journal of Physiology - Renal Physiology, Journal of Urology, and Neurourology and Urodynamics. While at SCCP, I have established a leading urological laboratory conducting translational research related to detrusor function and dysfunction. I have also established a strong research group, consisting of undergraduate and graduate students, technicians, postdoctoral fellows, and junior faculty. Using a number of novel approaches and concepts for cutting-edge advances in the field, my research program seeks to fill important gaps in our understanding of the regulatory mechanisms of urinary bladder smooth muscle function and the role of ion channels. My research group has identified and clarified novel regulatory pathways and mechanisms in the bladder that involve ion channels using state-of-the-art patch-clamp electrophysiology, confocal microscopy, imaging approaches, functional studies on smooth muscle contractility, pharmacological and molecular biological techniques. This has resulted in a series of peer-reviewed articles published in top ranking journals with each article representing a critically important advance in the field of urology and influencing urological research worldwide. In collaboration with Dr. Eric Rovner (Co-I, current proposal) from the MUSC Department of Urology, we have established a comprehensive program in human detrusor ion channel research with the goal of addressing questions related to urinary bladder function and dysfunction in humans. A major strength and unique aspect of our strategic program on human detrusor ion channel research is that my laboratory conducts this research in collaboration with a highly experienced cadre of physician-scientists. Together, we have assembled a multidisciplinary research team of investigators with diverse expertise. Thus, based on my extensive and diverse training in pharmacology, physiology, electrophysiology, molecular biology, and basic urological research, my ability to form solid and relevant research collaborations, I am well suited to have a leadership role in this Pilot Project grant.

 

Education

Doctor of Philosophy, Physiology 1997
University of Sofia, Sofia, Bulgaria

 

Research Highlight

Publications

1. Petkov, G.V. (2012). Role of potassium ion channels in detrusor smooth muscle function and dysfunction. Nature Reviews Urology, 9 (1): 30-40. (PMID: 22158596; doi: 10.1038/nrurol.2011.194; PMCID: PMC3759241). (Invited review).

2. Hristov, K.L., Chen, M., Soder, R.P., Parajuli, S.P., Cheng, Q., Kellett, W.F., and Petkov, G.V. (2012). KV2.1 and electrically silent KV channel subunits control excitability and contractility of guinea pig detrusor smooth muscle. American Journal of Physiology - Cell Physiology, 302 (2): C360-372. (PMID: 21998137; doi: 10.1152/ajpcell.00303.2010; PMCID: PMC3328844).

3. Afeli, S.A.Y., Hristov, K.L., and Petkov, G.V. (2012). Do β3-adrenergic receptors play a role in guinea pig detrusor smooth muscle excitability and contractility? American Journal of Physiology - Renal Physiology, 302 (2): F251-263. (PMID: 21993887; doi: 10.1152/ajprenal.00378.2011; PMCID: PMC3340921).

4. Parajuli, S.P., Soder, R.P., Hristov, K.L., and Petkov, G.V. (2012). Pharmacological activation of small conductance calcium-activated potassium channels with naphtho[1,2-d]thiazol-2-ylamine decreases guinea pig detrusor smooth muscle excitability and contractility. Journal of Pharmacology and Experimental Therapeutics, 340 (1): 114-123. (PMID: 22001258; doi: 10.1124/jpet.111.186213; PMCID: PMC3251021).

5. Xin, W., Cheng, Q., Soder, R.P., and Petkov, G.V. (2012). Inhibition of phosphodiesterases relaxes detrusor smooth muscle via activation of the large conductance voltage- and Ca2+-activated K+ channel. American Journal of Physiology - Cell Physiology, 302 (9): C1361-1370. (PMID: 22322973; doi: 10.1152/ajpcell.00432.2011; PMCID: PMC3361951).

6. Hristov, K.L., Chen, M., Afeli, S.A.Y., Cheng, Q., Rovner, E.S., and Petkov, G.V. (2012). Expression and function of KV2-containing channels in human urinary bladder smooth muscle. American Journal of Physiology - Cell Physiology, 302 (11): C1599-1608. (PMID: 22422395; doi: 10.1152/ajpcell.00447.2011; PMCID: PMC3378019).

7. Hristov, K.L., Parajuli, S.P., Soder, R.P., Cheng, Q., Rovner, E.S., and Petkov, G.V. (2012). Suppression of human detrusor smooth muscle excitability and contractility via pharmacological activation of large conductance Ca2+-activated K+ channels. American Journal of Physiology - Cell Physiology, 302 (11): C1599-C1608. (PMID: 22422396; doi: 10.1152/ajpcell.00417.201; PMCID: PMC3378020).

8. Afeli, S.A.Y., Rovner, E.S., and Petkov, G.V. (2012). SK but not IK channels regulate human detrusor smooth muscle spontaneous and nerve-evoked contractions. American Journal of Physiology - Renal Physiology, 303 (4): F559-568. (PMID: 22592639; doi: 10.1152/ajprenal.00615.2011; PMCID: PMC3423111).

9. Xin, W., Cheng, Q., Soder, R.P., Rovner E.S., and Petkov, G.V. (2012). Constitutively active phosphodiesterase activity regulates urinary bladder smooth muscle function: Critical role of KCa1.1 channel. American Journal of Physiology - Renal Physiology, 303 (9): F1300-1306. (PMID: 22896041; doi: 10.1152/ajprenal.00351.2012; PMCID: PMC3518192).

10. Xin, W., Soder, R.P., Cheng, Q., Rovner E.S., and Petkov, G.V. (2012). Selective inhibition of phosphodiesterase 1 relaxes urinary bladder smooth muscle: role for ryanodine receptor mediated BK channel activation. American Journal of Physiology - Cell Physiology, 303 (10): C1079-1089. (PMID: 22992675; doi: 10.1152/ajpcell.00162.2012; PMCID: PMC3492835).

 

2013

11. Parajuli, S.P., Hristov, K.L., Soder, R.P., Kellett, W.F., and Petkov, G.V. (2013). NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels. British Journal of Pharmacology, 168, 1611-1625. (PMID: 23145946; doi: 10.1111/bph.12049; PMCID: PMC3605870).

12. Soder, R.P., Parajuli, S.P., Hristov, K.L., Rovner, E.S., and Petkov, G.V. (2013). SK channel-selective opening by SKA-31 induces hyperpolarization and decreases contractility in human urinary bladder smooth muscle. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology, 304 (2): R155-R163. (PMID: 23174857; doi: 10.1152/ajpregu.00363.2012; PMCID: PMC3543661).

13. Smith A.C., Parajuli, S.P., Hristov, K.L., Cheng, Q., Soder, R.P., Afeli, S.A.Y, Earley, S., Xin, W., Malysz, J., and Petkov, G.V. (2013). TRPM4 channel: A new player in urinary bladder smooth muscle function in rats. American Journal of Physiology - Renal Physiology, 304 (7): F918-929. (PMID: 23283997; doi:10.1152/ajprenal.00417.2012; PMCID: PMC3625855).

14. Malysz, J., Rovner, E.S., Petkov, G.V. (2013). Single-channel biophysical and pharmacological characterizations of native human large conductance calcium-activated potassium channels in freshly isolated detrusor smooth muscle cells. Pflϋgers Arch – European Journal of Physiology, 465 (7), 965-975. (PMID: 23344746; DOI: 10.1007/s00424-012-1214-8; PMCID: PMC3659209).

15. Smith A.C., Hristov, K.L., Cheng, Q., Xin, W., Parajuli, S.P., Earley, S., Malysz, J., and Petkov, G.V. (2013). Novel role for the transient potential receptor melastatin 4 channel in guinea pig detrusor smooth muscle physiology. American Journal of Physiology - Cell Physiology, 304 (5): C467-477. (PMID: 23302778; doi: 10.1152/ajpcell.00169.2012; PMCID: PMC3602646).

16. Afeli, S.A.Y., Petkov, G.V. (2013). Functional BK channels facilitate the beta-3 adrenergic receptor-mediated relaxation of nerve-evoked contractions in rat urinary bladder smooth muscle isolated strips. European Journal of Pharmacology, 711 (1-3): 50-56 (PMID: 23643998; doi: 10.1016/j.ejphar.2013.04.020; PMCID: PMC3679346).

17. Parajuli, S.P., and Petkov, G.V. (2013). Activation of muscarinic M3 receptors inhibits large-conductance voltage- and Ca2+-activated K+ channels in rat urinary bladder smooth muscle. American Journal of Physiology - Cell Physiology, 305 (2): C207-214 (PMID: 23703523; doi: 10.1152/ajpcell.00113.2013; PMCID: PMC3725628).

18. Hristov, K.L., Afeli, S.A.Y., Parajuli S.P., Cheng Q., Rovner, E.S., Petkov, G.V. (2013). Neurogenic detrusor overactivity is associated with decreased expression and function of the large conductance voltage- and Ca2+-activated K+ channels. PLoS One, 8 (7), e68052 (PMID: 23861849; doi: 10.1371/journal.pone.0068052; PMCID: PMC3702577).

19. Afeli, S.A.Y., Rovner, E.S., Petkov, G.V. (2013). BRL37344, a β3-adrenergic receptor agonist, decreases nerve-evoked contractions in human detrusor smooth muscle isolated strips: role of BK channels. Urology 82 (3): 744.e1-7 (PMID: 23890664; doi: pii: S0090-4295(13)00686-9. 10.1016/j.urology.2013.05.027; PMCID: PMC3758792).

20. Afeli, S.A.Y., Malysz, J., Petkov, G.V. (2013). Molecular expression and pharmacological evidence for a functional role of Kv7 channel subunits in guinea pig urinary bladder smooth muscle. PLoS One 8(9): e75875 (PMID: 24073284; doi:10.1371/journal.pone.0075875; PMCID: PMC3779188).

21. Parajuli, S.P., Hristov, K.L., Sullivan, M.N, Xin, W., Smith A.C., Earley, S., Malysz, J., Petkov, G.V. (2013). Control of Urinary Bladder Smooth Muscle Excitability by the TRPM4 channel modulator 9-phenanthrol. Channels (Austin) 7 (6), 537-540 (PMID: 24037125; doi: 10.4161/chan.26289; PMCID: PMC4042489).

 

2014

22. Malysz, J., Afeli, S.A.Y., Provence, A., Petkov, G.V. (2014). Ethanol-mediated relaxation of guinea pig urinary bladder smooth muscle: involvement of BK and L-type Ca2+ channels. American Journal of Physiology - Cell Physiology, 306(1): C45-58 (PMID: 24153429; doi: 10.1152/ajpcell.00047.2013; PMCID: PMC3919972).

23. Hristov, K.L., Smith, A.C., Parajuli, S.P., Malysz, J., Petkov, G.V. (2014). Large conductance Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle. American Journal of Physiology - Cell Physiology 306(5): C460-70 (PMID: 24352333; doi: 10.1152/ajpcell.00325.2013; PMCID: PMC4098151).

24. Xin, W., Li., N., Cheng, Q., Petkov, G.V. (2014). BK channel-mediated relaxation of urinary bladder smooth muscle: A novel paradigm for phosphodiesterase type 4 regulation of bladder function. Journal of Pharmacology and Experimental Therapeutics, 349(1): 56-65. (PMID: 24459245; doi: 10.1124/jpet.113.210708; PMCID: PMC3965888).

Figure 1 of this article has been selected as the cover illustration for the April, 2014 issue of The Journal of Pharmacology and Experimental Therapeutics.

25. Parajuli, S.P., Provence, A., Petkov, G.V. (2014). Prostaglandin E2 excitatory effects on guinea pig urinary bladder smooth muscle: A novel regulatory mechanism mediated by large-conductance voltage- and Ca2+-activated K+ channels. European Journal of Pharmacology 738: 179-185 (PMID: 24886877; doi: 10.1016/j.ejphar.2014.05.042; PMCID: PMC4107080).

26. Petkov, G.V. (2014). Central role of the BK channel in urinary bladder smooth muscle physiology and pathophysiology. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 307: R571–R584 (PMID: 24990859: doi:10.1152/ajpregu.00142.2014; PMCID: PMC4166757).

27. Xin, W., Li., N., Cheng, Q., Fernandes, V. Petkov, G.V. (2014). Constitutive PKA activity is essential for maintaining the excitability and contractility in guinea pig urinary bladder smooth muscle: Role of the BK channel. American Journal of Physiology - Cell Physiology 307(12): C1142-C1150 (PMID: 25318105; doi: 10.1152/ajpcell.00167.2014; PMCID: PMC4269660).

28. Parajuli, S.P., Hristov, K.L., Cheng, Q., Malysz, J., Rovner, E.S., Petkov, G.V. (2015). Functional link between muscarinic receptors and large-conductance Ca2+-activated K+ channels in freshly-isolated human detrusor smooth muscle cells. Pflügers Archiv. - European Journal of Physiology 467: 665–675 (PMID: 24867682; doi: 10.1007/s00424-014-1537-8; PMCID: PMC4247359).